TRANSGENIC OIL PALM ANALYSIS VIA FLUORESCENCE in situ HYBRIDISATION, FLOW CYTOMETRY AND CYTOLOGICAL TECHNIQUES
نویسندگان
چکیده
منابع مشابه
Telomere analysis by fluorescence in situ hybridization and flow cytometry.
Determination of telomere length is traditionally performed by Southern blotting and densitometry, giving a mean telomere restriction fragment (TRF) value for the total cell population studied. Fluorescence in situ hybridization (FISH) of telomere repeats has been used to calculate telomere length, a method called quantitative (Q)-FISH. We here present a quantitative flow cytometric approach, Q...
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Fast and accurate analysis of fluorescence in-situ hybridisation (FISH) images depends upon two components: a classifier to discriminate between artefacts and valid signal data, and well-discriminating features to represent the signals. After processing the image, we evaluate candidate feature sets by illustrating the probability density functions and scatter plots for the features. This analys...
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Previous research has indicated the significance of accurate classification of fluorescence in situ hybridisation (FISH) signals for the detection of genetic abnormalities. Based on well-discriminating features and a trainable neural network (NN) classifier, a previous system enabled highly-accurate classification of valid signals and artefacts of two fluorophores. However, since this system em...
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Background: Most of our current understanding of the biological effects of exposure to ionising radiation is based on conventional cytogenetic techniques, which enable us to determine the relationship between chromosomal aberration and dose received by radiation workers. However, conventional techniques have numerous limitations and chromosomal aberrations can be easily missed. Since FISH plays...
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ژورنال
عنوان ژورنال: Journal of Oil Palm Research
سال: 2018
ISSN: 1511-2780
DOI: 10.21894/jopr.2018.0047